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2022 Vol.9, Issue 3 Preview Page

Original Article

Evaluation of Growth Inhibition for Microcystis aeruginosa with Ultrasonic Irradiation Time

초음파 조사시간에 따른 Microcystis aeruginosa의 성장억제 평가

Eun Byeol Kang1
,
Jin Chul Joo2*
,
So Ye Jang3
,
Hyeon Woo Go4
,
Jung Su Park5
,
Moo Il Jeong6
,
Dong Ho Lee7
강 은별1
,
주 진철2*
,
장 소예3
,
고 현우4
,
박 정수5
,
정 무일6
,
이 동호7
1Master Candidate, Department of Civil and Environmental Engineering, Hanbat National University, Daejeon 34158, Korea
2Professor, Department of Civil and Environmental Engineering, Hanbat National University, Daejeon 34158, Korea
3Master, Environmental Engineering, Hanbat National University, Daejeon 34158, Korea
4Master Candidate, Department of Civil and Environmental Engineering, Hanbat National University, Daejeon 34158, Korea
5Assistant Professor, Department of Civil and Environmental Engineering, Hanbat National University, Daejeon 34158, Korea
6CEO, Adsonic, Daejeon 34014, Korea
7Professor, Department of Mobile Convergence Engineering, Hanbat National University, Daejeon 34158, Korea
1한밭대학교 건설환경공학과 석사과정
2한밭대학교 건설환경공학과 교수
3한밭대학교 환경공학과 석사
4한밭대학교 건설환경공학과 석사과정
5한밭대학교 건설환경공학과 조교수
6㈜애드소닉 대표
7한밭대학교 모바일융합공학과 교수
*Corresponding Author
30 September 2022. pp. 183-193
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Abstract

The growth inhibitory effect of Microcystis aeruginosa according to the ultrasonic irradiation time was evaluated using a large algae sample volume (10 L) for various ultrasonic irradiation times (0.5, 1, 1.5, 2, 2.5 and 3 hr) at a laboratory scale. Based on the analysis of Chl-a and cell number of M. aerginosa, algae growth inhibition was observed with the decrease in Chl-a and cell number in all experimental groups after the ultrasonic irradiation. For the experimental group (T_B, T_C, T_D) with an ultrasonic irradiation time of less than 2 hours, rapid regrowth of algae was observed after growth inhibition, but the experimental group (T_E, T_F, T_G) with an irradiation time of more than 2 hours successfully inhibited algal growth lasting one or two more days. Based on the comparison of the recovery time to initial cell number the experimental group (T_B, T_C, T_D) took less than 20 days whereas the experimental group (T_E, T_F, T_G) took about 30 days. Correspondingly, the experimental group showed a high first order decay rate (κ) in proportion to the ultrasonic irradiation time during the growth inhibition period. Additionally, the specific growth rates (μ) during regrowth in the experimental group with irradiation time of more than 2 hours were relatively low compared to those in the experimental group with less than 2 hours. Therefore, ultrasonic irradiation for more than 2 hours is required for long-term (30 days) inhibition of algal growth in stagnant waters. However, the appropriate ultrasonic irradiation time for algae growth inhibition should be determined according to various field conditions such as the volume of stagnant water, water depth, flow rate, algae concentration, etc. Finally, damages to the algal cell surface and cell membrane were clearly observed, and both destruction and disturbance of gas vesicles of M. aeruginosa in the experimental group were discovered, indicating the growth inhibitory effect of Microcystis aeruginosa according to the ultrasonic irradiation time was confirmed.

Keywords
Algae growth inhibition
Irradiation time
Microcystis aeruginosa
Regrowth
Ultrasonic irradiation

초음파 (ultrasonic) 조사시간 (irradiation time)에 따른 Microcystis aeruginosa M. aeruginosa)의 성장억제 (growth inhibition) 효과를 대용량 (10 L) 조류 시료를 활용해 다양한 초음파 조사시간 동안 (0.5, 1, 1.5, 2, 2.5, 3 hr) 실험실 규모 (lab-scale) 실험을 진행하였다. Chl-a와 M. aeruginosa 개체수 (cell number) 분석 결과, 초음파 조사 종료 이후 모든 실험군에서 Chl-a와 개체수가 감소되어 조류 성장억제가 관찰되었으며, 초음파 조사시간이 2시간 미만인 실험군 (T_B, T_C, T_D)은 저감 이후 조류의 급격한 재성장 (regrowth)이 관측되었으나, 조사시간이 2시간 이상인 실험군 (T_E, T_F, T_G)은 2시간 미만 조사한 실험군 대비 조류성장억제가 1 - 2일 더 지속되는 것을 확인하였다. 또한, 조류의 개체수가 초기 개체수까지 회복 시간 (recovery time)을 검토한 결과 조사시간 2시간을 기준으로 실험군 T_B (0.5 hr)는 7일, T_C (1 hr)와 T_D (1.5 hr)는 약 20일, T_E, T_F, T_G (≥ 2 hr)는 약 30일이 소요되어 초음파 조사 종료 후 초기 개체수까지의 회복 시간의 차이를 확인하였다. 비성장속도 (μ)와 일차분해속도 (κ)를 도출한 결과, 실험군은 성장억제 기간 동안 초음파 조사시간과 비례하여 높은 일차분해율이 도출되었으며, 조사시간이 2시간 이상인 실험군의 재성장 시 비성장속도 (μ)는 2시간 미만인 실험군 대비 비성장속도 (μ)가 상대적으로 낮은 것으로 확인되었다. 따라서 정체수역 내 장기적인 (30일) 조류 성장억제를 위해서는 최소한 2시간 이상의 초음파 조사가 필요한 것으로 판단되나, 조류 성장억제를 위한 적정 초음파 조사시간은 정체수역의 규모, 수심, 흐름속도, 조류 농도 등의 다양한 현장조건에 따라 결정돼야 하며, 실제 적용 가능성을 높이기 위한 추가 연구가 필요한 것으로 판단된다. 초음파의 영향으로 인한 M. aeruginosa의 세포 표면 및 내부형질 변화를 관찰한 결과, 조류 세포 표면 및 세포막의 손상이 명확히 관측되었으며, 대조군 대비 실험군의 M. aeruginosa의 기낭 파괴 및 교란이 확인되었다.

키워드
조류 성장억제
조사시간
Microcystis aeruginosa
재성장
초음파 조사
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Information
  • Publisher :Korean Society of Ecology and Infrastructure Engineering
  • Publisher(Ko) :응용생태공학회
  • Journal Title :Ecology and Resilient Infrastructure
  • Journal Title(Ko) :응용생태공학회 논문집
  • Volume : 9
  • No :3
  • Pages :183-193
  • Received Date : 2022-09-01
  • Revised Date : 2022-09-12
  • Accepted Date : 2022-09-20
  • DOI :https://doi.org/10.17820/eri.2022.9.3.183
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